Equilibration with simple safeguard

Introduction	This section contains an example of how a Watch instruction for simple safeguard can be inserted into a method.

Example instruction

This is an example instruction as it would be presented in the Text pane:

0.00 Block EQUILIBRATE

(Equilibrate)

0.00
Base SameAsMain

0.00
Watch_UV1 Less_than, 100 {mAU} END_BLOCK

5.00
Watch_Off UV1

5.00
Message “The Condition was never reached”, Screen, “No sound”

5.00
End_Block

This is what happens

This is what happens in the above example:

The column is equilibrated until the UV has reached a level below 100 mAU or until the column has been equilibrated with five column volumes of buffer, whichever condition is met first. In this way, it is possible to equilibrate the column without the risk of running out of buffers.

2005-06-15