How to edit the Column List

Introduction

This section describes how to edit the list of available columns.

Available columns

When you create a new method and select a column, certain column-specific parameters are automatically copied into the method. The list of available columns is found in the For column field of the New Method dialog box. The Column List is not linked to a particular method, although the columns are edited within the Method Editor.

Columns are either globally available to all users, or only personally available. It is best not to edit the globally available columns, unless you save the changes under a new column name, since other users may not appreciate the changes.

Note: It is recommended that only a limited number of users are given access to the right to edit global columns. This is essential to avoid unintentional changes.

How to print the column list

The table below describes how to print the column list data.

Step

Action

1

  • Click the print button.

Result: The Print Column List dialog box opens.

2

  • Select to print a global or your personal column list.

  • Click OK.

Result: The column list is printed on the default Windows printer.

The New Column dialog box

The illustration below shows the New Column dialog box:

How to add a new column

The table below describes how to add a new column to the Column List.

Step

Action

1

  • Choose Edit:Column List in the Method Editor.

Result: The Column List dialog box opens.

Note: Select a column from the list to display the parameters in the field to the right. Most column parameters are displayed in the Normal Parameters tab. Additional parameters for special columns may be displayed in the Advanced Parameters tab.

2

  • Click the New button.

Result: The New Column dialog box opens.

3

  • Select the appropriate parameter tab.

  • Type the desired parameter values.

  • Click the Save as button.

Note: Mandatory parameters are labelled mand. The column cannot be saved unless all mandatory parameters are filled in.

Result: The Save as dialog box opens.

4

  • Type the name of the new column.

  • Click the Save as global checkbox if the column should be available to other users.

Note: You must have Edit global lists authorization to save a column for global use. A global column cannot have the same name as a personal column.

  • Click OK.

Result: The new column is added to the Column List.

Note: See column instruction to determine the back pressure over the system and the column.

The normal column parameters

The table below is a list of all the available normal column parameters:

Parameter

Unit

Comment

Height

cm

  • Mandatory.

  • Calculation of N/m.

Diameter

cm

  • Mandatory.

Column volume

nl, µl, ml or liter

  • Mandatory.

  • Automatically calculated from Height and Diameter.

  • User cannot set this parameter directly.

Column volume unit

nl, µl, ml or liter

  • Not mandatory.

  • The column volume is calculated in the set unit.

Technique

 

  • Mandatory.

  • Decides which technique the column should be available for.

Vt

nl, µl, ml or liter

  • Not mandatory.

  • Total liquid volume.

  • Used to calculate the capacity factor after an integration.

Vo

nl, µl, ml or liter

  • Not mandatory.

  • Void volume.

  • Used to calculate Kav after integration.

Max pressure

MPa

  • Mandatory.

  • Used for setting pressure limit in a method automatically.

Default flowrate

nl/min, µl/min, ml/min or liter/min

  • Mandatory.

  • Used to set the flowrate in a method automatically.

Max flowrate

nl/min, µl/min, ml/min or liter/min

  • Not mandatory.

  • Used to give a warning if a higher flowrate is chosen when saving or starting a method.

Typ. peak width at base

nl, µl, ml or liter

  • Not mandatory.

  • Used to set averaging time for UV detector.

  • used to set peak fractionation parameters.

pH high value, longterm

 

  • Not mandatory.

  • Used to give a warning when saving or starting the method if the BufferPrep_pH value is higher than the set value.

pH low value, longterm

 

  • Not mandatory.

  • Used to give a warning when saving or starting the method if the BufferPrep_pH value is lower than the set value.

pH high value, shortterm

 

  • Not mandatory.

  • Used to give a warning when saving or starting the method if the BufferPrep_pH value is higher than the set value.

pH low value, shortterm

 

  • Not mandatory.

  • Used to give a warning when saving or starting the method if the BufferPrep_pH value is lower than the set value.

Average particle diameter

µm

  • Not mandatory.

  • Information only.

Code no

 

  • Not mandatory.

  • Information only.

Typical loading range

mg

  • Not mandatory.

  • Information only.

Mol. weight range

kDa

  • Not mandatory

  • Information only

Scan rate

spectra/sec

  • Not mandatory.

  • Information only.

Note: The values for the parameters Max pressure, Default flowrate and Typical peak width at base (used to set average time and peak fractionation parameter MinWidth) are only copied into the method if the corresponding instructions are available as variables.

How to edit column parameters

The table below describes how to edit column parameters in the Method Editor:

Step

Action

1

Choose Edit:Column List.

Result: The Column List dialog box opens.

2

Select a column and click the Edit button.

Result: The Edit Column dialog box opens.

3

Select the desired parameters and change the value settings.

4

  • Click the Save button.

or

  • Click the Save as button to save the column under a new name.

Note: If a column has been selected and saved in a method, and the parameters for the column are changed later, the column in the method will not be updated automatically. When you open the method you will be asked if you want to update the parameters. The recommendation is that you answer Yes.

How to delete a column

The table below describes how to delete a column:

Step

Action

1

Choose Edit:Column List.

Result: The Column List dialog box opens.

2

Select a column and click the Delete button.

Result: The Delete Column dialog box opens.

3

  • Click the checkbox for each column you want to delete.

  • Click OK.

Result: The selected columns are deleted.

How to export a column

The column information for a system can be transferred to another by using the export and import functions in the column list. The table below describes how to export a column:

Step

Action

1

Choose Edit:Column List.

Result: The Column List dialog box opens.

2

Click the Export button.

Result: The Export Column dialog box opens.

3

  • Click the checkbox for each column you want to export.

  • Click Export.

Result: The Export Column to file dialog box opens.

4

  • Select the desired folder in the navigation window.

  • Type a new file name if neccessary.

  • Choose the type of file to export (column file or Excel file)

  • Click the Save button.

Result: The column file is saved and the dialog box closes.

Note: If a column is selected in the Column List when the Export Column dialog box is opened, this column will automatically be selected in the Export Column dialog box.

How to import a column

The table below describes how to import a column:

Step

Action

1

Choose Edit:Column List.

Result: The Column List dialog box opens.

2

Click the Import button.

Result: The Import Column dialog box opens.

3

  • Click the Browse button to locate the column file.

Result: The Import Column from file dialog box opens.

4

  • Select a column file.

  • Click Open.

Result: The Import Columns dialog box opens.

5

  • Select the columns to import from the list.

  • Select Import as global to add the columns to the global column list if desired.

  • Click Import.

Result: The selected columns are imported and available in the column list.

Note: Select Import as global to import the columns to the global column list.

2005-06-15