1

• Choose Operations:Pool.

Result: UNICORN™ will automatically pool suitable fractions. The pooled fractions are listed in a table below the chromatogram and the pooled peaks are numbered sequentially in the chromatogram.

Note: Only adjacent fractions will be pooled. The fraction numbers for each pool are listed in the table as a range in retention order, e.g. A6-A7 etc.

2

The pooled fractions can be adjusted manually:

To include or exclude adjacent fractions in a pool

• Click the numbered marker under the pool and drag the sideline.

To add more pools

• Click between the droplines under a fraction to create a new pool, and drag the sidelines to include more adjacent fractions.

To delete pools

• Click the numbered marker to select the pool and click the Delete button. Click the Delete All button to clear all pools.

To restore the pools created by UNICORN

• Click the Default Pool button.

3

Other curves can be selected for the operation:

• Select another source curve from the Source curve droplist and click the Default Pool button.

or

• Select another baseline curve from the Baseline droplist and click the Default Pool button.

or

• Select another fraction curve from the Fraction curve droplist and click the Default Pool button.

Result: The pooled fractions in the list are replaced by the pooled fractions for the selected curve.

1

• Choose Operations:Create Pool Fraction curve.

Result: The Create Pool Fraction Curve dialog box opens.

2

• Select a position where the curve will be stored from the Save curve in list.

• If needed, type a new name in the Curve name text box.

Note: The suggested curve name will have the default suffix POOL.

• Click the OK button.

Result: The Pool Fraction curve is displayed in the chromatogram.

1

• Choose Edit:Chromatogram Layout.

or

• Right-click in the chromatogram and choose Properties from the shortcut menu.

Result: The Chromatogram Layout dialog box opens.

2

• Select the Curve tab.

• De-select the check box for the original fraction curve (remove the check mark).

Result: The original fraction curve is de-selected and is not displayed.

1

• Open a result file in the Evaluation module.

• Pool fractions as described in How to pool fractions above.

• Click the Add to Pooling Protocol button.

Result: The pooled fractions from the active result file is added to the Pooling Protocol.

2

• Repeat step 1 to add pooled fractions from other result files.

Note: You will be asked to save the current file when you open the next. The pool table will not be saved.

3

• Click the View Pooling Protocol button.

Result: The Pooling Protocol dialog box opens.

4

• Click the Show all fractions checkbox to display the individual fractions instead of fraction ranges for the pools.

• Click the Show all columns checkbox to display all the information columns from the Pool table.

Possible actions in the Pooling Protocol

To delete a single pool...

• select a pool and click the Delete button

To clear the whole protocol...

• click the Delete all button.

To print the protocol on the default Windows™ printer...

• click the Print button to print the protocol on the default Windows printer.

To export the protocol...

• click the Export button to save the protocol in one of the following formats:

  text (.txt)

  Excel (.xls)

  HTML (.htm)

  XML (.xml)

Note: The protocol is automatically saved for the user. The pooling protocol will be available again when the user starts UNICORN the next time.

5

• Click the Close button to close the Pooling Protocol dialog box.

Result: If the protocol was exported or only edited, the dialog box will close. If the protocol was printed, a dialog box will open asking if you want to delete the list and start a new.