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How to set up a Scouting Scheme
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Introduction
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This section describes how to set up a method for scouting.
Any parameter can be scouted, provided that it can be defined
as a variable in the method.
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When to use scouting
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Scouting
is a facility for automatically repeating a run with systematic
variation of one or more parameters. Some typical situations where
scouting is useful are when you want:
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to screen for the
best column
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to find the optimal pH
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to test column capacity (sample volume)
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to find the optimal flow rate for binding and elution
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to optimize gradient length and slope
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to optimize step gradients.
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Variable values
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The
variables that appear in the scouting scheme are usually a subset
of those on the Variables tab
of the Run Setup. The values
in the scouting scheme can only be set on the Scouting tab, while the default
values in the method can be set either on the Variables tab or in the Text instruction pane.
Changing
variable values in the scouting scheme does not change the values on the Variables tab or in the text instructions.
Values for variables selected for scouting are grey on the Variables tab and cannot be changed
there.
Any changes that you make to variable values when a scouting
scheme is run are saved in the result file. Results from a scouting
run are saved in a scouting folder.
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Scouting tab buttons
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There
are seven buttons on the Scouting tab
of the Run Setup plus the Help button. The table describes
the functions of these buttons:
Click the button...
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if you want...
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Define...
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to define new scouting variables. The Scouting Variables dialog box
is displayed, and you can select variables to be used in the scouting
series.
Note: The variables
that have been selected for scouting cannot be
changed on the Variables tab.
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Clear All
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to clear all runs.
This converts the scouting run to a non-scouting run so that
it contains only the original method and values.
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Delete
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to remove a run from the Scouting tab.
Click on any variable in the run you want to remove, and then
click the Delete button.
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Insert
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to insert a new scouting run before an existing run.
Click on a run column and then click the Insert button. The new run will
inherit the variable values from
or
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Add
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or
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Series...
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to set up a series of runs with differing inputs.
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Edit Variable...
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to rename or delete a variable, or change a variable into
a detail variable.
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How to set up or edit a Scouting Scheme
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The
table below describes how to set up or edit a scouting scheme.
Step
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Action
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1
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Create a method. If you do not use a template or wizard,
define appropriate variables in the method.
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2
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In the Run Setup,
click the Scouting tab.
Result: If no scouting
variables have been previously defined, the Scouting
Variables dialog box is displayed. If not, click the Define button.
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3
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Select the variables you want to scout. If you cannot
find the variable you want, use the following options:
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Show details to display variables
created with the Visible in details
only option.
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Show unused variables to
display all variables, including those that are not used in the
method.
Click OK.
Result: The selected
scouting variables will appear in a column, with default valued
inserted.
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4
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Make any required changes in the scouting variable
values.
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5
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To add a new Run column,
click the Add button to
copy the values from the last run column, and then change variable
values as required.
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6
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Repeat steps 3 to 5 as required until you have defined
all the scouting runs you need.
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7
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To exclude scouting runs from the default scouting
scheme, right-click the heading of the run. To include the scouting
run again, right-click it again.
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8
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Click the Start Protocol tab
in Run Setup.
Select from the following options:
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The Scouting box: Select this to display
the Scouting page at the
start of a run. This allows the operator to adjust the values for scouting
variables before the method run starts.
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The First run only button:
Select this to display the start protocol before the first run only.
The settings entered in the Start Protocol for
the first run will apply throughout the run, and the scouting series
will be performed automatically without user intervention.
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The All runs button:
Select this to display the Start Protocol before
each run in the scheme. This gives the operator an opportunity to
change variable values or fill the sample loop before each run.
Note: The operator
must then click the Start button
before each run.
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How to set up series
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The table below describes how to set up series.
Step
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Action
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1
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Select a cell on the Scouting tab,
and click the Series button.
Result: The Insert Series dialog box is displayed.
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2
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In the Insert Series dialog
box, type the selected series values (within the specified range
limits), separated by commas, and click OK.
Result: A new set
of runs is inserted on the Scouting tab
with the values provided.
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How to delete or rename scouting variables
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Scouting
variables can be deleted or renamed in the scouting scheme in the
same way as in the Variables tab.
The table below describes how to delete or rename a variable in
the Scouting tab.
Step
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Action
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1
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Click the Edit Variable... button
on the Scouting tab.
Result: The Edit Variables dialog box opens.
The variables are listed alphabetically.
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2
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Select the variable to edit.
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3
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Rename
Result: The variable
is renamed.
Delete
Result: The variable
is deleted.
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How to change a scouting variable into a detail variable
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Detail
variables are indicated with a D to
the left of the Variable column
on the Scouting tab. The table below describes
how to set up a detail variable.
Step
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Action
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1
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Click the Edit Variable... button
on the Scouting tab.
Result: The Edit Variables dialog box opens.
The variables are listed alphabetically.
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2
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Choose the variable to be changed.
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3
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Result: The variable
is indicated by a D.
Note: De-select
the checkbox to make the variable fully visible again.
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How to copy contents to factorial design programs
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The contents
of the Scouting tab can
be copied and pasted into a third-party factorial design program.
Processed values can then be pasted back into the Scouting tab. The table below
describes how to do this:
Step
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Action
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1
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Select the text, etc., that you want to copy.
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2
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Press Ctrl+C.
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3
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Place the cursor where you want to insert the copied
text.
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4
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Press Ctrl+V.
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2005-06-15
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