How to use the Fraction Histogram

Introduction

The Fraction Histogram dialog box in the Evaluation module can be used to create a curve for the average fraction absorbance.

How to create a Fraction Histogram

The table below describes how to create a Fraction Histogram curve.

Step

Action

1

Select Operations:Fraction histogram.

Result: The Fraction histogram dialog box opens.

2

  • Select the desired UV curve.

Note: The fractions curve should already be selected on the middle list. If you have previous pooled fractions and created a pooled fraction curve, select the desired fraction curve.

3

  • Click OK.

Result: The average fraction absorbance values are displayed as a new curve in the chromatogram.

2005-06-15