1

Perform a run with one or more standards to create a standard curve.

Note: The standards should contain a number of components of known molecular size and these should extend beyond the size limits that are expected in the test sample.

2

Peak integrate the standard curve to produce a peak table.

3

Use the peak table from the standard to produce a molecular size table. Each peak is represented by a retention value.

4

Select the relevant peaks and input data for the corresponding molecular sizes.

Result: The software plots these values as a molecular size curve. This curve has an inverse relationship between the logarithm of the molecular size and retention.

1

Use the sample peak table to obtain retention values for each of the components of interest.

2

Use the molecular size curve to obtain the molecular sizes of the components in the sample. The molecular sizes are presented in the peak table.