1

Perform two runs.

2

Copy the curves into one result file.

3

Integrate the curves to produce the peak tables.

4

Select the component to be used.

5

Evaluate the amount of a component in the sample.

1

Perform a sample run with the unspiked sample and a run with the spiked sample.

2

Open one of the two result files. Use File:Open:Curves to copy the second curve to the opened result file.

3

Peak integrate the sample curves to produce the peak tables for the unspiked and the spiked samples.

Note: The sample curves must use the same X-axis base unit. Time is the recommended unit for highest reliability.

4

• Check that the integrations are correct.

• Optimize the peak integration if necessary.

5

Select File:Save to save the peak table.

1

Select Quantitate:Standard addition

Result: The Standard Addition dialog box opens.

2

• Select the chromatogram that contains the peak table for the unspiked sample in the Source chromatogram droplist.

• Select the unspiked sample peak table from the Peak table(s) list to the left.

3

Repeat step 2 in the Addition chromatogram section to the right to select the addition peak table for the spiked sample.

4

• Edit the default unit mg in the Unit label field if necessary.

• Type the amount of the component that was added as the spike in the Added amount field.

• Click OK.

Result: The Identify Peak dialog box opens.

5

To locate and select the peak of the unspiked sample, do the following:

• Click its triangle marker (black) or select its reference in the Source table.

6

• Repeat step 5 to select the spiked sample. The triangle color is blue. Use the Addition table.

• Click the OK button.