The Gradient tab

The Gradient tab provides a graphical overview of the block structure and eluent gradient in the current method. The description of this tab can also serve as a description of the Gradient pane of the Text Instructions.

Note: For scouting runs, click Run X to see the gradient for each run.

The illustration below shows an example of a Gradient tab:

The table below describes how to zoom in on a selected area of the Gradient tab:

To reduce the scale of the zoom function, right-click the tab and choose either:

• Undo Zoom to reverse each zoom-in action a step at a time, or

• Reset Zoom to reverse all of the zoom-in actions to the default scale setting.

A vertical marker line can be dragged from the Y-axis with the mouse. As you drag the marker line, the current position is identified at the top of the tab in terms of the block name, X-position in the currently displayed base and eluent concentration in per cent of eluent B.

You can change the base shown on the Gradient X-axis. The alternatives are time, volume and column volumes. Changing the base for the display does not affect the base in the method instructions, which means that you can check how long a method will take simply by setting the axis scale to time, even if the method blocks are written in volume or column volume base.

The list below describes two ways to change the base shown on the X-axis:

• Click the X-axis to toggle between the base types.

or

• Right-click anywhere on the Gradient tab.

  Result: A sub-menu is displayed.

• Select Base and make the appropriate choice: Time, Volume or CV.

You can display a hatched background on the Gradient tab. The table below describes how to do this: