How to perform automated quantitation


Instruction

The table below describes how to set up sample runs to perform automated quantitation.

Step

Action

1

2

  • Click the Scouting tab in the Run Setup.

  • Click the Clear All button to clear the scouting scheme.

  • Double-click each Quantitation_Type table cell and select Sample for all sample runs.

3

Click the Evaluation Procedures tab.

  • Select only the Quantitate_Sample procedure. (Click the Import button to import the Quantitate_Sample procedure if it isn’t displayed on the list)

Result: This procedure automatically integrates the first UV curve with default baseline settings and uses the selected quantitation table to quantitate the sample. The amounts and concentrations are then printed.

4

Click the Quantitate button.

Result: The Quantitation table dialog box opens.

5

Select the quantitation table from the Global or Personal folder and click OK.

Result: The quantitation table is copied into the Quantitate_Sample procedure.

Note: The procedure cannot be executed if a quantitation table has not been selected. Time must have been selected as the X-axis base unit.

6

Save the method with a new name.

7

Perform the run(s).

Result: The amount and concentration of the components in the samples will be printed automatically after each run.


2005-06-15