How to set up for automated quantitation

Introduction

This section describes how to create a quantitation table for automated quantitation.

Basic conditions for the quantitation table

A quantitation table must be produced from standards before samples can be quantitated. The list below describes the basic conditions for the quantitation table:

  • The same method must be used for all standard and sample runs.

  • Each level is an alias for a specific concentration of the standard.

  • All runs with the same concentration must be assigned the same level.

  • Level 1 must be selected for the standard with the highest or lowest concentration.

  • The levels must be set in order of decreasing or increasing concentration of the standard.

How to prepare the quantitation table

The table below describes how to prepare the quantitation table for automated quantitation.

Step

Action

1

  • Use the Method Wizard to create a method.

  • Select Autosampler from the Injection Technique droplist in the Sample Injection dialog box.

2

Proceed with the following dialog boxes in the Method Wizard and click the Finish button on the last dialog box.

Result: The Run Setup opens.

3

Click the Scouting tab. (See illustration below)

  • Select the Quantitation_Type variable from the Scouting Variables dialog box.

  • Select other scouting variables of interest, e.g. Sample_ID, Vial_Number etc.

  • Click OK.

4

  • Double-click the Quantitation_Type variable table cell.

  • Select the correct standard concentration level.

Note: This corresponds to the text instruction QuantitationData. You can also set this level after the run has been completed. For more information about scouting see How to set up a scouting scheme.

5

Click the Evaluation Procedures tab.

  • Select the Integrate_and_Print procedure.

Result: This procedure will automatically use default baseline settings and integrate the first UV curve.

6

Save the method.

7

Perform all the standard runs.

8

In the Evaluation module, select Quantitate:Edit Quantitation Table:New.

9

Create a quantitation table manually from the standard runs. See How to create a quantitation table.

The Scouting tab

The illustration below shows the Scouting tab in the Run Setup, used to enter standard data, before the standard concentration level is defined.

2005-06-15