1

Open a method in the Method Editor.

2

• Click the Scouting tab in the Run Setup.

• Click the Clear All button to clear the scouting scheme.

• Double-click each Quantitation_Type table cell and select the correct concentration level for the standards.

3

Click the Evaluation Procedures tab.

• Select the Update_Quantitation procedure. (Click the Import button to import the Update_Quantitation procedure if it isn’t displayed on the list)

Result: This procedure automatically integrates the first UV curve with default baseline settings and updates the selected quantitation table with the new standard. An update report is then printed.

4

Click the Quantitate button.

Result: The Quantitation table dialog box opens.

5

Select the quantitation table from the Global or Personal folder. Time must be selected as the X-axis base unit.

Result: The quantitation table is copied into the Update_Quantitation procedure.

Note: You can only perform one run at each level since the old points in the quantitation table will be replaced after each run.

6

Save the method with a new name.

7

Perform the run(s).

Result: The quantitation table will be updated automatically after each run.

1

Open a method in the Method Editor.

2

• Click the Scouting tab in the Run Setup dialog box.

• Click the Clear All button to clear the scouting scheme.

• Double-click each Quantitation_Type table cell and select the correct concentration level for the standards.

3

• Click the Evaluation Procedures tab.

• Select the Update_Quantitation procedure.

• Click the Quantitate button.

Result: The Quantitation table dialog box opens.

4

Select the quantitation table from the Global or Personal folder and click OK.

Result: The quantitation table is copied into the Update_Quantitation procedure.

5

Click the Edit button on the Evaluation Procedures tab.

Result: The Procedure Editor dialog box opens. See illustration below.

6

Select the existing UPDATE instruction.

7

Use the scroll bar in the Parameter field to locate the Average or replace point droplist.

• Select the AVERAGE option.

• Click the Replace button to the right of the scroll bar.

8

Select File:Close in the Procedure Editor dialog box to return to the Run Setup.

9

Save the method and perform the runs.

Result: The quantitation table will be updated automatically after each run. New average values will be calculated from the old points together with the new points.

1

Open the same method that was used to create the quantitation table from the standard runs and open the Run Setup.

2

Click the Evaluation Procedures tab.

3

Select Update_Quantitation and click Quantitate.

Result: The Quantitation table dialog box opens.

4

Select the quantitation table and click OK.

5

Deselect the Update_Quantitation procedure on the Evaluation Procedures tab.

6

Repeat steps 3 to 5 for the Quantitate_Sample procedure.

Note: Make sure that both procedures are deselected after this is completed. Otherwise they will be run twice.

7

Proceed with the instructions how to edit the instructions (see table below).

1

Click the Text Instructions icon.

2

Select the last instruction in the method in the Text pane.

3

• Click the Other radio button in the Instructions field of the Instruction box.

• Select Evaluate on the Instructions list.

4

• Select Update_Quantitation in the Procedure droplist of the Parameters field.

• Click the Var... button in the Parameters field.

Result: The Variable Name Definition dialog box opens.

5

Type a variable name, for example Procedure and click OK.

Result: The Evaluate instruction is inserted in the method. By defining the evaluation procedure as a variable, different procedures can be selected on the Scouting tab for different scouting runs.

6

Proceed with the instructions on how to set up the scouting runs for the standards (see table below).

1

Select View:Run Setup and click the Scouting tab.

2

Click the Define button.

Result: The Scouting Variables dialog box opens.

3

Edit the scouting variables list to include:

• Procedure

• Vial_Number

• Injection_volume

• Sample_ID

• Quantitation_Type

Note: The Procedure variable will appear at the beginning of the list of variables, even though the Evaluate instruction is inserted at the end of the method.

4

Set up all the standard runs in the scouting scheme:

• Select the Update_Quantitation procedure.

• Ensure that Quantitation_Type is set to the correct standard level for each run.

Result: The quantitation table will now be updated with new values after each run. Since the runs will be performed with the Replace (the default selection) option, you can only perform one run at each level.

5

Proceed with the instructions on how to set up the scouting runs for the samples in step 4 (see table below).

1

Select the Quantitate_Sample procedure.

2

Select Sample in the variable Quantitation_Type for all the sample runs.

Result: All samples will be run automatically and the amount and concentration of the components of interest will be printed after each run.

Note: The result files will include an additional chromatogram (labelled 12) that contains a small part of the curves collected during the execution of the evaluation procedure.

1

• Click the Evaluation Procedures tab in the Run Setup Editor.

• Click the Import button.

Result: The Import dialog box opens.

2

• Select the current method from the Method file menu.

• Highlight Update_Quantitation in the Select field.

• Type a new name, e.g. Update_Average in the Import as text box.

• Click the Import button.

• Click the Close button.

Result: The new procedure is added to the list of Evaluation Procedures.

3

Select the new procedure and click the Edit button.

Result: The Procedure Editor dialog box opens.

4

Highlight the existing Update instruction.

5

Use the scroll bar in the Parameter field to locate the Average or replace point droplist.

• Select the AVERAGE option.

• Click the Replace button.

6

Select File:Close in the Procedure Editor dialog box to return to the Run Setup.

7

Select the Update_Average procedure and click the Quantitate button.

Result: The Quantitation table dialog box opens.

8

Select the quantitation table and click OK.

9

Deselect all the procedures on the Evaluation Procedures tab, otherwise they will be run twice.

10

Click the Scouting tab.

• Select the Update_Average procedure for the second and all following runs at each standard level concentration.

Note: The Update_Quantitation procedure (Update by Replace) should still be used for the first run at each level.