SMA
For details see individual articles.
|
Cell line
|
Description
|
Reference
|
Used in
|
|---|---|---|---|
|
NIH/3T3-VEGFR-2
|
mouse embryonic fibroblasts stably transfected with VEGFR-2
|
(Waltenberger et al. 1994)
|
III
|
|
NIH/3T3-VEGFR-3
|
mouse embryonic fibroblasts stably transfected with VEGFR-3
|
(Pajusola et al. 1993)
|
III
|
|
Sf-9
|
fall army worm (Spodoptera frigiperda) ovarian cells
|
Invitrogen
|
II, III
|
|
High Five
|
cabbage looper (Trichoplusia ni) ovarian cells
|
Invitrogen
|
II, III
|
|
S2
|
fruit fly (Drosophila melanogaster) cells
|
Invitrogen
|
IV
|
|
Ba/F3-VEGFR-1-EpoR
|
mouse pre-B lymphocytes stably transfected with a VEGFR-1-EpoR chimera
|
(Makinen et al. 2001b)
|
IV
|
|
Ba/F3-VEGFR-2-EpoR
|
mouse pre-B lymphocytes stably transfected with a VEGFR-2-EpoR chimera
|
(III; Stacker et al. 1999b)
|
IV
|
|
Ba/F3-VEGFR-3-EpoR
|
mouse pre-B lymphocytes stably transfected with a VEGFR-3-EpoR chimera
|
(Achen et al. 2000)
|
IV
|
|
293T
|
human kidney epithelial cells expressing the SV40 T antigen and the transforming gene of adenovirus 5
|
American Type Culture Collection CRL-1573
|
IV
|
|
PAE-VEGFR-2
|
porcine aortic endothelial cells stably transfected with VEGFR-2
|
(Waltenberger et al. 1994)
|
IV
|
|
PAE-VEGFR-3
|
porcine aortic endothelial cells stably transfected with VEGFR-3
|
(Pajusola et al. 1994)
|
IV
|
| Recombinant protein | Description1 |
Reference
|
Used in
|
|---|---|---|---|
|
VEGF-C
|
mature form
|
II, III
|
II, IV
|
|
full length form
|
III
|
III
|
|
|
avian mature form
|
IV
|
IV
|
|
|
mature VEGFR-3-specific mutant
|
IV
|
IV
|
|
|
109 amino acid VHD
|
IV
|
IV
|
|
|
VEGF-D
|
full length and mature forms
|
III
|
III
|
|
VEGF-A
|
165 amino acid isoform of VEGF-A
|
IV
|
IV
|
|
109 amino acid VHD
|
IV
|
IV
|
|
|
VEGF-B
|
186 amino acid isoform
|
IV
|
IV
|
|
VEGF-E
|
viral strains NZ7 and NZ2
|
IV
|
IV
|
|
HSA
|
serum albumin
|
IV
|
IV
|
|
VEGF/VEGF-C mosaics
|
109 amino acid VHD, produced in both 293T and High Five cells
|
IV
|
IV
|
|
VEGFR-1D1-3-hIgGFC
|
first three extracellular domains of VEGF receptor-1 fused to the FC domain of human IgG, produced in S2 cells
|
(Makinen et al. 2001a) |
IV
|
|
VEGFR-2D1-3-hIgGFC
|
first three extracellular domains of VEGF receptor-2 fused to the FC domain of human IgG, produced in 293T cells
|
III
|
IV
|
|
VEGFR-3D1-3-hIgGFC
|
first three extracellular domains of VEGF receptor-1 fused to the FC domain of human IgG, produced in 293T cells
|
(Makinen et al. 2001a)
|
IV
|
|
Antigen
|
Description
|
Reference
|
Used in
|
|---|---|---|---|
|
vWF
|
monoclonal antibody against human van Willebrand factor
|
DAKO
|
IV
|
|
SMA
|
monoclonal antibody against alpha smooth muscle actin
|
Sigma
|
IV
|
|
Prox-1
|
rabbit antiserum against human Prox-1
|
(Papoutsi et al. 2000)
|
IV
|
|
DNA vectors
|
Description
|
Reference
|
Used in
|
|---|---|---|---|
|
pFASTBAC1
|
baculoviral transfer vector
|
Invitrogen
|
III
|
|
pFB1-melSP-H6
|
baculoviral transfer vector based on pFASTBAC1, modified by addition of the honeybee melittin signal peptide and a hexahistidine tag
|
II, III, IV
|
II, III, IV
|
|
pSecTagI
|
dual mammalian/bacterial expression vector based on pSecTagA (Invitrogen); modified by insertion of a bacterial promoter, a tailored MCS and deletion of several restriction sites in the backbone
|
IV
|
IV
|
|
pKO Scrambler V912
|
minimal multi-purpose cloning vector
|
Stratagene
|
IV
|
|
pIgplus, Signal pIgplus
|
for secreted expression in mammalian cells as a fusion protein with the FC domain of human IgG
|
Ingenius
|
IV
|
|
pMT/BiP/V5-His C
|
for inducible, secreted expression in S2 cells
|
Invitrogen
|
IV
|
|
Methods
|
Used in
|
|---|---|
|
Generation of transgenic mice
|
I
|
|
Recombinant production and purification of proteins using insect and mammalian cells
|
II, III, IV
|
|
Receptor phosphorylation assay
|
III, IV
|
|
DNA family shuffling
|
IV
|
|
Ba/F3 proliferation assay
|
IV
|
|
CAM assay
|
IV
|
|
Immunohistochemistry
|
IV
|
|
Molecular modeling
|
IV
|