1	Introducing UNICORN
	1.1	About UNICORN
	1.2	About this manual
	1.3	About the UNICORN user documentation

2	UNICORN concepts
	2.1	Concept definitions
	2.2	The UNICORN user interface
	2.2.1	UNICORN Manager
	2.2.2	The Method Editor module
	2.2.3	The System Control module
	2.2.4	The Evaluation module
	2.2.5	Search functions
	2.2.6	Help functions and manuals
	2.2.7	Snapshots
	2.3	Quick Start Guide

3	General system operations
	3.1	Log on routines and log off routines
	3.2	How to create a new user
	3.3	How to assign user properties
	3.4	How to change your passwords and user attributes
	3.5	How to connect to the chromatography system
	3.6	How to back up and restore system data
	3.7	How to set up a printer

4	Files and folders in UNICORN
	4.1	How to create folders
	4.2	How to open and preview files
	4.3	How to arrange and locate your files
	4.4	How to copy, delete, rename and backup files and folders

5	How to create a method
	5.1	How to use the Method Wizard
	5.2	How to use the Method templates
	5.3	How to use Text instructions
	5.4	How to sign the method

6	How to edit methods
	6.1	The Method Editor interface
	6.1.1	Method Editor module
	6.1.2	Text Instructions editor
	6.2	Method blocks
	6.2.1	How to view method blocks
	6.2.2	How to call method blocks
	6.2.3	How to add method blocks
	6.2.4	How to delete method blocks
	6.2.5	How to rename method blocks
	6.2.6	How to find, copy and move method blocks
	6.2.7	How to import method blocks
	6.3	Method instructions
	6.3.1	How to read method instructions
	6.3.2	How to add method instructions
	6.3.3	How to delete method instructions
	6.3.4	How to change or move method instructions
	6.4	How to use method variables
	6.5	Run Setup
	6.5.1	Overview of Run Setup
	6.5.2	The Variables tab
	6.5.3	The Scouting tab
	6.5.4	The Questions tab
	6.5.5	The Gradient tab
	6.5.6	The Notes tab
	6.5.7	The Evaluation Procedures tab
	6.5.8	The Reference Curves tab
	6.5.9	The Columns tab
	6.5.10	The BufferPrep tab
	6.5.11	The Method Information tab
	6.5.12	The Result Name tab
	6.5.13	The Frac-950 tab
	6.5.14	The Start Protocol tab
	6.5.15	How to export the values in the Run Setup
	6.6	How to use selected method instructions
	6.6.1	Base instruction
	6.6.2	Instructions at the same breakpoint
	6.6.3	Block and method length
	6.6.4	Messages and Set_Marks
	6.6.5	How to delay a method
	6.6.6	Linear flow rates
	6.6.7	Gradients and eluent concentrations
	6.7	Standard Watch conditions
	6.8	How to save or delete a method template
	6.9	How to print a method
	6.10	How to export a method

7	Scouting
	7.1	How to set up a Scouting Scheme
	7.2	How to define different columns for scouting

8	MethodQueues
	8.1	How to create a new MethodQueue
	8.2	How to edit a MethodQueue

9	How to perform method runs
	9.1	How to start a method run
	9.2	How to monitor a method run
	9.2.1	How to customize System Control panes
	9.2.2	The Run Data pane
	9.2.3	The Curves pane
	9.2.4	The Flow Scheme pane
	9.2.5	The Logbook pane
	9.3	Manual system control
	9.3.1	The toolbar and status bar
	9.3.2	Manual instructions
	9.3.3	Alarms and warnings
	9.4	How to perform a scouting run
	9.5	How to perform a MethodQueue run
	9.6	If the network connection fails

10	How to view results
	10.1	How to open a result file
	10.2	How to use the File Navigator
	10.3	Basic presentation of chromatograms
	10.3.1	Introduction and temporary chromatograms
	10.3.2	The chromatogram window
	10.4	How to optimize the presentation of a chromatogram
	10.4.1	How to make changes in the Chromatogram Layout dialog box
	10.4.2	The Curve tab and Curve Names tab
	10.4.3	The Curve Style and Color tab
	10.4.4	How to change and fix the axes
	10.4.5	How to save and apply a layout
	10.4.6	How to show part of a curve
	10.4.7	How to change the size of Fraction, Injection and Logbook marks
	10.5	How to print active chromatograms
	10.6	How to create and print reports
	10.6.1	How to create and print a customized report
	10.6.2	How to create and print a standard report
	10.6.3	How to edit an existing report format
	10.7	Run documentation

11	How to edit results
	11.1	How to reduce noise and remove ghost peaks
	11.2	How to subtract a blank run curve
	11.3	How to add curves
	11.4	How to enter and edit text in the chromatogram
	11.5	How to pool fractions
	11.6	How to match protein activity to a curve
	11.7	How to rename chromatograms, curves and peak tables
	11.8	How to import and compare different runs
	11.8.1	How to use the Multifile Peak Compare wizard
	11.8.2	How to import and compare chromatograms
	11.8.3	How to import and compare curves
	11.8.4	How to stack and stretch curves
	11.8.5	How to produce a mirror image
	11.9	How to import and export results
	11.9.1	How to import results
	11.9.2	How to export results
	11.10	How to sign results electronically
	11.11	How to save results and exit the Evaluation module

12	Evaluation
	12.1	Peak integration
	12.1.1	Baseline calculation
	12.1.2	How to perform a peak integration
	12.1.3	How to optimize the baseline with a morphological algorithm
	12.1.4	How to optimize the baseline with a classic algorithm
	12.1.5	How to edit the baseline manually
	12.1.6	How to edit the peaks
	12.1.7	How to integrate part of a curve and how to exclude or skim peaks
	12.1.8	Measurements
	12.2	Other evaluations
	12.2.1	Peak purity and peak identification
	12.2.2	How to find slope values
	12.2.3	How to simulate a peak fractionation
	12.2.4	How to create curves
	12.2.5	How to use the Fraction Histogram
	12.3	Automated evaluation procedures
	12.3.1	How to create a new procedure
	12.3.2	How to edit a procedure
	12.3.3	How to run a procedure
	12.3.4	How to rename and remove procedures

13	The Analysis module
	13.1	General information about the module
	13.2	Quantitation overview
	13.2.1	General information about quantitation
	13.2.2	External standard quantitation
	13.2.3	Internal standard quantitation
	13.2.4	Standard addition quantitation
	13.2.5	Recovery calculation
	13.2.6	General reliability factors for the quantitation techniques
	13.3	How to prepare for quantitation
	13.3.1	Preparations before quantitation
	13.3.2	How to create a quantitation table
	13.3.3	How to edit and update a quantitation table
	13.4	How to quantitate the sample
	13.4.1	External and internal standard quantitation
	13.4.2	Standard addition quantitation
	13.4.3	How to calculate the recovery factor
	13.5	Automated quantitation
	13.5.1	How to set up for automated quantitation
	13.5.2	How to perform automated quantitation
	13.5.3	How to perform automated update
	13.6	How to measure molecular size
	13.6.1	Overview of molecular size determination
	13.6.2	How to determine the molecular size

14	System settings
	14.1	General information about system settings
	14.2	Alarms
	14.3	Curves

15	System maintenance and error reporting
	15.1	System maintenance functions
	15.2	How to generate problem reports
	15.2.1	How to generate a report from the UNICORN Manager
	15.2.2	How to generate a report from the System Control

A	Troubleshooting
	A.1	Logon
	A.2	UNICORN access
	A.3	Methods and method runs
	A.4	Evaluation
	A.5	ÄKTAdesign system specific problems

B	Evaluation functions and instructions
	B.1	Smoothing algorithms
	B.2	Baseline calculation theory
	B.3	Peak table column components
	B.4	Procedure instructions

C	Curve fit models and statistics
	C.1	Curve fit models
	C.2	Statistics

D	The Column list
	D.1	How to edit the Column List

E	How to create and edit BufferPrep recipes
	E.1	How to create a BufferPrep recipe
	E.2	How to edit a BufferPrep recipe

F	Method examples
	F.1	Simple equilibration
	F.2	Equilibration with simple safeguard
	F.3	Equilibration with extra safeguard
	F.4	Collection of absorbance peaks
	F.5	Collection of three absorbance peaks
	F.6	Messages
	F.7	Quality control procedure

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